CXCL10 Inhibitors

ABSTRACT

Provided herein are compounds and compositions effective for inhibiting CXCL10 gene expression, production, and secretion in mammalian cells, tissues and organs, as well as ameliorating its biological activity, along with a method for treatment of one or more disorders associated with an increase in CXCL10 gene expression, production, and secretion.

This Nonprovisional application claims priority under 35 U.S.C. § 119(e)on U.S. Provisional Application No. 63/017,137 filed on Apr. 29, 2020,the contents of which are hereby incorporated by reference in itsentirety.

FIELD OF THE INVENTION

This invention pertains to inhibitors of the production of CXCL10 andamelioration of its biological activity on mammalian cells and tissues.The invention provides compounds and methods useful for inhibition ofthe biological effects of CXCL10 on mammalian cells and for thetreatment of human diseases associated with increased levels of CXCL10.

BACKGROUND OF THE INVENTION

CXCL10 is a small molecular weight inducible chemokine that has beenfound to be present in high levels in a variety of human diseases.CXCL10 is not normally produced by mammalian cells and tissues however.Certain types of normal mammalian and human cells, such as endothelial,epithelial, fibroblast, keratinocyte, smooth muscle, mesangial,astrocyte, mononuclear, and microglial cells, can be induced to produceand secrete high levels of CXCL10 by stimulation with various substancesassociated with infectious agents, such as lipopolysaccharide (LPS). Inaddition, a variety of stress conditions and substances that mimicbacterial, viral and fungal components, such as Poly (I:C), induceproduction of CXCL10 in normal cells. In certain diseases, immune,metabolic, neoplastic disorders and cancers CXCL10 is produced inrelatively large amounts and is present in high levels in the blood andother tissue fluids in humans suffering from these disorders.

CXCL10 binds to and transduces signals through its receptor CXCR3. Thisreceptor is present on many types of human cells such as, but notlimited to: leukocytes, peripheral blood mononuclear cells (PBMCs),fibroblasts, vascular smooth muscle cells, endothelial cells, andastrocytes (Vazrinejad R, et al. Neuroimmunomodulation 2014;21:322-330). The variety of cells that express CXCR3 and mediate CXCL10biological effects implicates CXCL10 in many human disorders, such as:arthritis, asthma, cancer, dermatitis, endometriosis, lichen planus,psoriasis, Sjogren syndrome, uveitis, and others. However, CXCL10 mayalso produce biological effects through non-CXCR3 dependent mechanisms.

Increased CXCL10 production by human endothelial, epithelial,fibroblasts, keratinocytes, smooth muscle, mesangial, astrocytic,monocytic, microglial, or retinal cells and the like promotes diseaseinitiation and/or progression, subsequent tissue destruction, and lossof function in various human tissues such as, but not limited to, thedermis, epidermis, ocular uvea, macula, retina, pulmonary bronchial, andurogenital epithelium. In addition, inherited genetic variants in theCXCL10 gene region are associated with several diseases and promotesusceptibility to diseases. Therefore, CXCL10 is an importanttherapeutic target to treat various diseases such as those referencedherein. There are no approved drugs on the market that reduce cellularproduction of CXCL10. Thus, there is a need for chemical compounds thatinhibit the production of CXCL10 by mammalian cells.

SUMMARY OF THE INVENTION

As described herein the inventor has discovered that compounds embodiedby Formula I inhibit the gene expression, production and secretion ofthe bioactive protein CXCL10 from mammalian cells, tissues and organs.In certain embodiments this disclosure provides compounds that aretherefore capable of inhibiting the subsequent biologic effects elicitedby CXCL10 on mammalian cells, tissues and organs and methods oftreatment, prevention, inhibition or amelioration of one or morediseases associated with CXCL10 using the compound and compositionsdisclosed herein.

According to one embodiment of the present invention, there is providedinhibition of the production of CXCL10 by mammalian cells, tissues andorgans comprising the topical application, either alone or incombination with other bioactive substances, of at least one compoundaccording to Formula 1 below:

wherein:

X is selected from CH or N;

Y is selected from CH₂, CHOH, C(optionally substituted C₁ to C₈ straightchain or branched chain alkyl)OH, C═O, NH, N-optionally substituted C₁to C₈ straight chain or branched chain alkyl, NCO-optionally substitutedC₁ to C₈ straight chain or branched chain alkyl, S, S═O, SO₂;

R₁, R₂, R₃, R₄, R₅, R₆, and R₇ are independently selected from: H; OH;F; Cl; Br; I; (halogen)alkyl, optionally substituted C₁ to C₈ straightchain or branched chain alkyl; optionally substituted C₁ to C₈cycloalkyl; heterocycloalkyl; alkylheterocycloalkyl; optionallysubstituted C₁ to C₈ alkenyl; optionally substituted C₁ to C₈ alkynyl;optionally substituted aryl; optionally substituted alkylaryl;optionally substituted heteroaryl; optionally substitutedalkylheteroaryl; O-alkyl; O-optionally substituted alkyl, O-cycloalkyl;O-alkylcycloalkyl; O-aryl; O-optionally substituted aryl; alkyl-O-aryl;alkyl-O-optionally substituted aryl; C(O)-aryl; C(O)-optionallysubstituted aryl; CH₂C(O)-aryl; CH₂C(O)-optionally substituted aryl;O-(halogen)alkyl, or adjacent substituents R₁ and R₂, R₂ and R₃, R₄ andR₅, R₅ and R₆, R₆ and R₇, may form a saturated or unsaturated 5 memberedor 6-membered or 7 membered carbocyclic or heterocyclic ring, andoptionally substituted alkenyl, if present, may have one or more doublebond and each double bond may independently be cis or trans, E or Z, acis/trans mixture or an E/Z mixture, wherein if an asymmetric center ispresent or asymmetric centers are present the compound may be in theform of a racemic mixture, a single enantiomer, a diastereoisomericmixture, an enantiomeric diastereomer, a meso compound, a pure epimer,or a mixture of epimers thereof, and a hydrogen, several hydrogens orall hydrogens may be replaced with deuterium or the compound is apharmaceutically acceptable salt, ester or prodrug form thereof.

An additional embodiment of the invention is the inhibition of thebiological effects of CXCL10 on mammalian cells such as, but not limitedto, epithelial cells, mononuclear cells, as well as human tissues byapplication to these cells or tissues with a compound or compoundsaccording to Formula 1, either alone or in combination with otherbioactive substances, a physiological pH.

A further embodiment of the invention is the use of a compound asdefined above in the manufacture of a medicament for the treatment of amammal at risk for or having at least one disease or disorder associatedwith elevated CXCL10 levels. Non-limiting examples of such disordersare: skin disorders and/or diseases; urticarial conditions; respiratoryailments; airway and pulmonary conditions; ocular disorders;genito-urinary disorders; neurologic disorders, neoplastic disorders,cancers, infection-based diseases; fibrotic disorders, and trauma andtissue injury-based conditions.

BRIEF DESCRIPTIONS OF THE DRAWINGS

FIG. 1a-b : Decreased gene expression and cellular production of CXCL10by human keratinocyte cells after treatment with compounds of Formula 1.Cells were stimulated with Poly (I:C) and the relative gene expressionof CXCL10 (FIG. 1b ) and induced production and secretion of CXCL10(FIG. 1b ) were measured relative to controls cells not treated with thecompounds of Formula 1.

FIG. 2a-b : Decreased gene expression and cellular production of CXCL10by human bronchial epithelial cells after treatment with compounds ofFormula 1. Cells were stimulated with Interferon gamma (IFN-7) and therelative gene expression of CXCL10 (FIG. 2a ) and induced production andsecretion of CXCL10 (FIG. 2b ) were measured relative to controls cellsnot treated with the compounds of Formula 1.

FIG. 3a-b : Decreased gene expression and cellular production of CXCL10by human peripheral blood mononuclear cells after treatment withcompounds of Formula 1. Cells were stimulated with Lipopolysaccharide(LPS) and the relative gene expression of CXCL10 (FIG. 3a ) and inducedproduction and secretion of CXCL10 (FIG. 3b ) were measured relative tocontrols cells not treated with the compounds of Formula 1.

FIG. 4a-b : Decreased gene expression and production of CXCL10 byreconstructed full thickness human epithelium after treatment withcompounds of Formula 1. Cells were stimulated with Interferon gamma(IFN-7) plus Tumor Necrosis Factor alpha (TNF-α) and the relative geneexpression of CXCL10 (FIG. 4a ) and induced production and secretion ofCXCL10 (FIG. 4b ) were measured relative to controls tissues not treatedwith the compounds of Formula 1.

FIG. 5a-b : Reduction in CXCL10 levels in retina tissue and reduction inretinal vascular permeability in diabetic rats treated with compoundsaccording to Formula 1. FIG. 5a presents a graph of CXCL10 proteinlevels as measured by ELISA. FIG. 5b presents a graph of retinalvascular permeability factor as a measurement of [concentration of EvansBlue dye in retina]/[concentration of Evans Blue dye in plasma xcirculation time].

FIG. 6: Reduction in dermatitis/eczema lesions in human subjects treatedwith compound according to Formula 1. Subjective score based onInvestigator's Global Assessment (IGA).

FIG. 7: Reduction in Topical Endoscopic Fundal Imaging (TEFI) score ofmice with experimental autoimmune uveoretinitis (EAU) treated with acompound according to Formula 1 verses vehicle control alone.

DETAILED DESCRIPTION OF INVENTION

Compounds embodied by Formula I inhibit the gene expression, productionand secretion of the bioactive protein CXCL10 from mammalian cells,tissues and organs. In certain embodiments this disclosure providescompounds that are therefore capable of inhibiting the subsequentbiologic effects elicited by CXCL10 on mammalian cells, tissues andorgans and methods of treatment, prevention, inhibition, or ameliorationof one or more diseases associated with CXCL10 using the compounds andcompositions disclosed herein.

Compounds embodied by Formula I may have one or several asymmetriccenters and therefore can exist in different stereoisomericconfigurations. Consequently, the compounds of Formula I can occur asindividual (pure) enantiomers, individual pure enantiomericdiastereomers as well as a mixture of enantiomers or diastereomers. Thescope of the present invention includes both single enantiomers andmixtures thereof in all ratios. The scope of the present inventionfurther includes all tautomeric forms (“tautomers”) of the compounds ofFormula I, and all mixtures thereof in any ratio. It will be appreciatedby one skilled in the art that a single compound may exhibit more thanone type of isomerism.

The enantiomeric compounds of Formula I may be resolved into their pureenantiomers by methods known to those skilled in the art, for example byformation of diastereoisomeric salts which may be separated, forexample, by crystallization; formation of diastereoisomeric derivativesor complexes which may be separated, for example, by crystallization,gas-liquid or liquid chromatography; selective reaction of oneenantiomer with an enantiomer-specific reagent, for example enzymaticesterification; or gas-liquid or liquid chromatography in a chiralenvironment, for example on a chiral support with a bound chiral ligandor in the presence of a chiral solvent. It will be appreciated thatwhere the desired stereoisomer is converted into another chemical entityby one of the separation procedures described above, a further step isrequired to liberate the desired enantiomeric form. Alternatively,specific stereoisomers maybe synthesized by using an optically activestarting material, by asymmetric synthesis using optically activereagents, substrates, catalysts or solvents, or by converting onestereoisomer into the other by asymmetric transformation or inversion.

The compounds of the present invention may exist in unsolvated as wellas a variety of solvated forms with pharmaceutically acceptable solventssuch as water, ethanol, and the like. In general, the solvated forms areconsidered equivalent to the unsolvated forms for the purposes of thepresent invention. It should be understood that pharmaceuticallyacceptable solvents further includes isotopically substituted solventssuch as D₂O, dimethyl sulfoxide-d6 and the like. The term ‘solvate’ isused herein to describe a complex comprising the compound of theinvention and one or more pharmaceutically acceptable solvent molecules,including water. As such, all manner of hydrates of the compound areincluded by the term ‘solvate’. It is intended that the presentinvention embrace unsolvated forms, solvated forms and mixtures ofsolvated forms in any ratio.

The compounds of the present invention and/or its salts and/or solvatemay exist as amorphous solids or may exist in one or more crystallinestates, i.e. polymorphs. Polymorphs of the compound of Formula I areencompassed in the present invention and may be prepared bycrystallization under a number of different conditions such as, forexample, using different solvents or different solvent mixtures;crystallization at different temperatures; and using various modes ofcooling ranging from very fast to very slow during crystallization.Polymorphs may also be obtained by heating or melting a compound ofFormula I followed by gradual or fast cooling. The presence ofpolymorphs may be determined by solid NMR spectroscopy, IR spectroscopy,differential scanning calorimetry, powder x-ray diffraction or othertechniques. It should be understood that all such crystalline andamorphous forms of the compound of Formula I, and its salts, solvatesand prodrugs thereof are encompassed by the invention and the claims.

The present invention also includes all pharmaceutically acceptableisotopically-labeled variations of the compound of Formula I. Suchisotopically-labeled variations are compounds having the same Formulaand molecular formula as the compound of Formula I but wherein one ormore atoms are replaced by atoms having an atomic mass or mass numberdifferent from the atomic mass or mass number usually found in nature.Examples of isotopes that may be incorporated into the compound of thepresent invention include isotopes of hydrogen, carbon, fluorine,nitrogen, and oxygen, such as 2H, 3H, 11C, 13C, 14C, 18F, 13N 15N 17Oand 18O, respectively.

Certain isotopically labeled variations of the compound of the presentinvention such as, for example, those incorporating a radioactiveisotope such as 3H and 14C, are useful in drug and/or substrate tissuedistribution studies. Tritium, i.e. 3H, and carbon-14, i.e. 14C, areparticularly preferred due their ease of preparation and detection.Further, substitution with heavier isotopes such as deuterium, i.e. 2H,can afford certain therapeutic advantages resulting from greatermetabolic stability, for example, increased in vivo half-life or reduceddosage requirements, and hence may be preferred in some circumstances.Isotopically labeled compounds of Formula I of this invention andprodrugs thereof can generally be prepared by carrying out theprocedures disclosed in the Schemes and/or in the Examples bysubstituting a readily available isotopically labeled reagent for anon-isotopically labeled reagent.

The compounds of Formula I may be administered as a prodrug. The termprodrug refers to a compound which is transformed in vivo to a compoundof Formula I, or a pharmaceutically acceptable salt or solvate of thecompound. The transformation may occur by various mechanisms, such asvia hydrolysis in blood. A prodrug of the compound of Formula I may beformed in a conventional manner according to methods known in the art. Athorough discussion of prodrugs is provided by V. Stella in Pro-drugs asNovel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series (Stella(1975)), and in Bioreversible Carriers in Drug Design (Roche (1987)),both of which are incorporated herein by reference.

“Alkyl” means a straight or branched chain, saturated hydrocarbonradical. By way of example, the hydrocarbon chain may have from one totwenty carbons, one to sixteen carbons, one to fourteen carbons, one totwelve carbons, one to ten carbons, one to eight carbons, one to sixcarbons, one to four carbons, etc. “Lower alkyl” may refer to alkylshaving, e.g., one to six carbons, one to four carbons, etc. In certainexamples, a straight chain alkyl may have from one to six carbon atomsand a branched alkyl three to six carbon atoms, e.g., methyl, ethyl,propyl, 2-propyl, butyl (including all isomeric forms), pentyl(including all isomeric forms), and the like. “Me” means methyl, “Et”means ethyl, and “iPr” means isopropyl. Alkyl may be optionallysubstituted, e.g., optionally substituted with oxygen, silicon, sulphuror optionally substituted with OH, O-alkyl, SH, S-alkyl, NH2, NH-alkyl.In another example, alkyl may be C1 to C12 straight chain or branchedchain alkyl optionally substituted with oxygen, silicon, sulphur oroptionally substituted with OH, O-alkyl, SH, S-alkyl, NH2, NH-alkyl.

“Alkylene” means a divalent alkyl, with alkyl as defined above.

“Aryl” means a monocyclic or bicyclic aromatic hydrocarbon radical,e.g., having from of 6 to 20 or 6 to 10 ring atoms e.g., phenyl ornaphthyl. Aryl may be optionally substituted, e.g., substituted phenylor substituted naphthyl.

“Alkylaryl” means a (alkylene)-R radical where R is aryl as definedabove. Alkylaryl may be optionally substituted. In certain examples,alkylaryl may be alkylphenyl, alkylsubstituted phenyl, alkylnaphthyl oralkylsubstituted naphthyl.

“Alkenyl” means a straight or branched chain, saturated hydrocarbonradical which contains a carbon-carbon double bond. By way of example,the hydrocarbon chain may have from two to twenty carbons, two tosixteen carbons, two to fourteen carbons, two to twelve carbons, two toten carbons, two to eight carbons, two to six carbons, two to fourcarbons, etc. “Lower alkenyl” may refer to alkenyls having, e.g., two tosix carbons, two to four carbons, etc. In certain examples, a straightchain alkenyl may have from two to six carbon atoms and a branched alkylthree to six carbon atoms, e.g., a vinyl group, an allyl group, butene(including all isomeric forms), pentene (including all isomeric forms),and the like. Alkenyl may be optionally substituted. In certainexamples, alkenyl may be a C2 to C12 straight chain or branched chainhydrocarbon containing a carbon-carbon double bond, optionallysubstituted with oxygen, silicon or sulphur or optionally substitutedwith OH, O-alkyl, SH, S-alkyl, NH2 or NH-alkyl.

“Alkynyl” means a straight or branched chain, saturated hydrocarbonradical which contains a carbon-carbon triple bond. By way of example,the hydrocarbon chain may have from two to twenty carbons, two tosixteen carbons, two to fourteen carbons, two to twelve carbons, two toten carbons, two to eight carbons, two to six carbons, two to fourcarbons, etc. “Lower alkynyl” may refer to alkynyls having, e.g., two tosix carbons, two to four carbons, etc. In certain examples, a straightchain alkynyl may have from two to six carbon atoms and a branched alkylthree to six carbon atoms, e.g., an acetylene group, a propargyl group,butyne (including all isomeric forms), pentyne (including all isomericforms), and the like. Alkynyl may be optionally substituted. In certainexamples, alkynyl may be a C2 to C12 straight chain or branched chainhydrocarbon containing a carbon-carbon triple bond, optionallysubstituted with oxygen, silicon or sulphur or optionally substitutedwith OH, O-alkyl, SH, S-alkyl, NH2 or NH-alkyl.

“Cycloalkyl” means a cyclic saturated or partially saturated hydrocarbonradical (or an alicyclic radical). By way of example, the cycloalkyl mayhave from three to twenty carbon atoms, from three to sixteen carbonatoms, from three to fourteen carbon atoms, from three to twelve carbonatoms, from three to ten carbon atoms, from three to eight carbon atoms,from three to seven carbon atoms, from three to six carbon atoms, etc.,wherein one or two carbon atoms may be replaced by an oxo group, e.g.,admantanyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl,cyclohexenyl, indanyl and the like.

“Alkylcycloalkyl” means a (alkylene)-R radical where R is cycloalkyl asdefined above; e.g., cyclopropylmethyl, cyclobutylmethyl,cyclopentylethyl, or cyclohexylmethyl, and the like. In another example,alkylcycloalkyl has four to twelve carbon atoms, i.e., C4-C12alkylcycloalkyl.

“O-alkyl” means an (oxygen)-R radical where R is alkyl as defined above.For example, O-alkyl may be an oxygen atom bonded to a C1 to C6 straightchain or branched chain alkyl.

“O-cycloalkyl” means an (oxygen)-R radical where R is cycloalkyl asdefined above. For example, O-cycloalkyl is an oxygen atom bonded to aC3 to C7 cycloalkyl.

“O-alkylcycloalkyl” means an (oxygen)-R radical where R isalkylcycloalkyl as defined above. For example, O-cycloalkyl is an oxygenatom bonded to a C4 to C8 alkylcycloalkyl.

“Heterocyclyl” or “heterocycloalkyl” means a saturated or unsaturatedmonocyclic group, in which one or two ring atoms are heteroatom selectedfrom N, O, or S, the remaining ring atoms being C. Heterocyclyl andheterocycloalkyl includes, e.g., where the heterocycle comprises one ortwo hetero atoms selected from O, S, or N, including a C2 to C6heterocycloalkyl. The heterocyclyl ring is optionally fused to a (one)aryl or heteroaryl ring as defined herein. The heterocyclyl ring fusedto monocyclic aryl or heteroaryl ring is also referred to in thisApplication as “bicyclic heterocyclyl” ring. Additionally, one or tworing carbon atoms in the heterocyclyl ring can optionally be replaced bya —CO— group. More specifically the term heterocyclyl includes, but isnot limited to, pyrrolidino, piperidino, homopiperidino,2-oxopyrrolidinyl, 2-oxopiperidinyl, morpholino, piperazino,tetrahydropyranyl, thiomorpholino, and the like. When the heterocyclylring is unsaturated it can contain one or two ring double bonds. Whenthe heterocyclyl group contains at least one nitrogen atom, it is alsoreferred to herein as heterocycloamino and is a subset of theheterocyclyl group. When the heterocyclyl group is a saturated ring andis not fused to aryl or heteroaryl ring as stated above, it is alsoreferred to herein as saturated monocyclic heterocyclyl.

“Alkylheterocycloalkyl” means an -(alkylene)-R radical where R isheterocyclyl ring as defined above e.g., tetraydrofuranylmethyl,piperazinylmethyl, morpholinylethyl, and the like. Alkylheterocycloalkylalso includes, e.g., where the heterocycle comprises one or two heteroatoms selected from O, S, or N and has three to eleven carbon atoms,i.e., C3 to C11 alkylheterocycloalkyl, and includes when N is present inthe heterocyclic ring the nitrogen atom may be in the form of an amide,carbamate or urea.

“Heteroaryl” means a monocyclic or bicyclic aromatic radical, where oneor more, preferably one, two, or three, ring atoms are heteroatomselected from N, O, or S, the remaining ring atoms being carbon.Representative examples include, but are not limited to, pyrrolyl,thienyl (thiophenyl), thiazolyl, imidazolyl, furanyl, indolyl,isoindolyl, oxazolyl, isoxazolyl, diazolyl, pyrazolyl, triazolyl,benzothiazolyl, benzoxazolyl, quinolinyl, isoquinolinyl, pyridinyl(pyridyl), pyrimidinyl, pyrazinyl, pyridazinyl, tetrazolyl, and thelike. Heteroaryl may be optionally substituted.

“Oxo” or “carbonyl” means a ═(O) group or C═O group, respectively.

The term “substituted” means that the referenced group is substitutedwith one or more additional group(s) individually and independentlyselected from groups described herein. In some embodiments, an optionalsubstituent is selected from oxo, halogen, —CN, —NH2, —OH, —NH(CH3),—N(CH3)2, alkyl (including straight chain, branched and/or unsaturatedalkyl), substituted or unsubstituted cycloalkyl, substituted orunsubstituted heterocycloalkyl, fluoroalkyl, substituted orunsubstituted heteroalkyl, substituted or unsubstituted alkoxy,fluoroalkoxy, —S-alkyl, —S(O)2-alkyl, —CONH((substituted orunsubstituted alkyl) or (substituted or unsubstituted phenyl)), —CON(Hor alkyl)2, —OCON(substituted or unsubstituted alkyl)2,—NHCONH((substituted or unsubstituted alkyl) or (substituted orunsubstituted phenyl)), —NHCOalkyl, —N(substituted or unsubstitutedalkyl)CO(substituted or unsubstituted alkyl), —NHCOO(substituted orunsubstituted alkyl), —C(OH)(substituted or unsubstituted alkyl)2, and—C(NH2)(substituted or unsubstituted alkyl)2. In some embodiments, byway of example, an optional substituent is selected from oxo, fluorine,chlorine, bromine, iodine, —CN, —NH2, —OH, —NH(CH3), —N(CH3)2, —CH3,—CH2CH3, —CH(CH3)2, —CF3, —CH2CF3, —OCH3, —OCH2CH3, —OCH(CH3)2, —OCF3,—OCH2CF3, —S(O)2-CH3, —CONH2, —CONHCH3, —NHCONHCH3, —COCH3, —COOH andthe like. In some embodiments, substituted groups are substituted withone, two or three of the preceding groups. In some embodiments,substituted groups are substituted with one or two of the precedinggroups. In some embodiments, substituted groups are substituted with oneof the preceding groups. Further, unless stated to the contrary, aformula with chemical bonds shown only as solid lines and not as wedgesor dashed lines contemplates each possible isomer, e.g., each enantiomerand diastereomer, and a mixture of isomers, such as racemic or scalemicmixtures.

“Addition compound” refers to a complex of two or more completemolecules in which each preserves its fundamental structure and nocovalent bonds are made or broken (for example, hydrates of salts,adducts).

“Aliphatic acid” refers to acids of nonaromatic hydrocarbons. Examplesof aliphatic acids include, but are not limited to, butyric acid,hexanoic acid, propionic acid, octanoic acid, and acetic acid.

“Alkene” refers to an unsaturated linear divalent hydrocarbon moiety ofone to twelve, typically one to six, carbon atoms or a saturatedbranched divalent hydrocarbon moiety of three to twelve, typically threeto six, carbon atoms. Exemplary alkene groups include, but are notlimited to, methylene, ethylene, propylene, butylene, pentylene, and thelike.

“Antagonist” refers to a compound or a composition that attenuates theeffect of an agonist. The antagonist can directly bind reversibly orirreversibly to a region of the receptor in common with an agonist. Anantagonist can also bind at a different site on the receptor or anassociated ion channel. Thus, the term “antagonist” includes afunctional antagonist. A “functional antagonist” refers to a compoundand/or composition that reverses the effects of an agonist by meansother than acting at the same receptor as the agonist, i.e., afunctional antagonist causes a response in the tissue or animal whichopposes the action of an agonist. Examples include agents which haveopposing effects on an intracellular second messenger or on aphysiologic state in an animal (for example, blood pressure).

“Biological activity” as used herein means having an effect on oreliciting or preventing a response from a living cell, tissue, organ orphysiologic activity, such as, but not limited to, altering gene and/orprotein expression, protein phosphorylation, cellular behavior, and/ororgan function.

“Biomarker” as used herein means a measurable indicator of the severityor the presence of a particular disease state. More generally abiomarker is anything that can be used as an indicator of a particulardisease state or some other physiological state of an organism.

“Carboxyl” refers to an organic functional group consisting of a carbonatom double bonded to an oxygen atom and single bonded to a hydroxylgroup.

“Chiral center” (i.e., stereochemical center, stereocenter, orstereogenic center) refers to an asymmetrically substituted atom, e.g.,a carbon atom to which four different groups are attached. The ultimatecriterion of a chiral center, however, is nonsuperimposability of itsmirror image. If an asymmetric center is present in one or moresubstituents, the compound may be in the form of a racemic mixture, asingle enantiomer, a diastereoisomeric mixture, an enantiomericdiastereomer, a meso compound, a pure epimer, or a mixture of epimersthereof.

“Derivative” refers to a compound that is derived from some parentcompound where one atom is replaced with another atom or group of atomsand usually maintains its general structure. For example,trichloromethane (chloroform) is a derivative of methane.

“Dermatitis” as used herein refers to a general or localizedinflammation of the skin, either due to an inherent skin defect, directcontact with an irritating substance, virus, bacteria, animal parasite,fungus or to an allergic reaction. Symptoms of dermatitis include:redness, itching, exudations, pain, fissures, cracks, ulcers, and insome cases blistering of the skin.

“Eczema” as used herein refers to an inflammatory condition of the skincharacterized by redness, itching, and oozing vesicular lesions whichbecome scaly, crusted, or hardened.

“Epithelium (epithelia, plural) or epithelial tissues” as used hereinmeans a type of animal tissue made up of densely packed cells that reston a basement membrane to act as a covering of a free surface such as,but not limited to the surface of the human body; or lining of variousbodily surfaces, such as but not limited to the eyes; or lining variousbody cavities such as but not limited the abdominal cavity; or liningthe lumina of tubular structures within organs, such as but not limitedto the respiratory epithelium, urogenital epithelium.

“Enantiomeric excess” refers to the difference between the amounts ofenantiomers. The percentage of enantiomeric excess (% ee) can becalculated by subtracting the percentage of one enantiomer from thepercentage of the other enantiomer. For example, if the % of(R)-enantiomer is 99% and % of (S)-enantiomer is 1%, the % ee of(R)-isomer is 99%-1% or 98%.

The terms “halo,” “halogen” and “halide” are used interchangeably hereinand refer to fluoro, chloro, bromo, or iodo.

“Haloalkyl” refers to an alkyl group as defined herein in which one ormore hydrogen atom is replaced by the same or different halo atoms. Theterm “haloalkyl” also includes perhalogenated alkyl groups in which allalkyl hydrogen atoms are replaced by halogen atoms. Exemplary haloalkylgroups include, but are not limited to: —CH₂F, —CH₂Cl, —CF3, —CH₂CF3,—CH₂CCl3, and the like.

“Hetero-substituted alkyl” refers to an alkyl group, as defined herein,that contains one or more heteroatoms such as N, O, or S. Suchheteroatoms can be hydroxy, alkoxy, amino, mono-, di-alkyl amino, thiol,alkylthiol, etc.

“Hydroxyalkyl” refers to an alkyl group, as defined herein, having oneor more hydroxyl substituent(s).

As used herein, the term “inhibiting” and grammatical equivalentsthereof refer to a decrease, limiting, and/or blocking of a particularaction, production, function, or interaction. In one embodiment, theterm refers to reducing the level of a given output or parameter to aquantity (e.g., the production of a biological active molecule) which isat least 40%, or less than the quantity in a corresponding uninhibitedcontrol. A reduced level of a given output or parameter need not,although it may, mean an absolute absence of the output or parameter.The invention does not require, and is not limited to, methods thatwholly eliminate the output or parameter. Substantial inhibition can beat least 40% inhibition of the production or biological effect.

“Keto acid” refers to organic compounds that contain a carboxylic acidgroup and a ketone group.

“Leaving group” has the meaning conventionally associated with it insynthetic organic chemistry, i.e., an atom or a group capable of beingdisplaced by a nucleophile and includes halo (such as chloro, bromo, andiodo), alkanesulfonyloxy, arenesulfonyloxy, alkylcarbonyloxy (e.g.,acetoxy), arylcarbonyloxy, mesyloxy, tosyloxy,trifluoromethanesulfonyloxy, aryloxy (e.g., 2,4-dinitrophenoxy),methoxy, N, O-dimethylhydroxylamino, and the like.

“Leukocyte or leukocytes” as used herein refer to a colorless cell thatcirculates in the blood and body fluids and is involved in counteractingforeign substances and infectious disease as well as being causative ofinflammatory diseases. They have also been referred to as white (blood)cells. There are several types, all are amoeboid cells with a nucleus,including lymphocytes, granulocytes, mononuclear, and macrophages.

“Ligand” as used herein means a biochemical substance in the form of anucleic acid, protein or peptide that forms a complex with anotherbiomolecule in a cell or tissue to serve a biological purpose.

“Moderate” as used herein means to decrease or increase the quality,quantity, intensity or duration of a biological product or process.

“Parenteral” as used herein means a route of administration of asubstance to an animal that occurs by other than by way of thegastrointestinal tract or alimentary canal, such as topical, intravenous(IV) injections and IV infusions.

“Peripheral blood mononuclear cell (PBMC)” as used herein means anyperipheral blood cell having a round nucleus. These cells are a subsetof leukocytes and consist of lymphocytes (T cells, B cells, NK cells)and mononuclear, whereas erythrocytes and platelets have no nuclei andgranulocytes (neutrophils, basophils, and eosinophils) have multi-lobednuclei.

“Pharmaceutically acceptable excipient” refers to an excipient that isuseful in preparing a pharmaceutical composition that is generally safe,non-toxic, and neither biologically nor otherwise undesirable, andincludes excipients that are acceptable for veterinary use as well ashuman pharmaceutical use.

“Pharmaceutically acceptable salt” of a compound means a salt that ispharmaceutically acceptable and that possesses the desiredpharmacological activity of the parent compound. Such salts include:acid addition salts formed with inorganic acids such as hydrochloricacid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, andthe like; or formed with organic acids such as acetic acid, propionicacid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvicacid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid,fumaric acid, tartaric acid, citric acid, benzoic acid,3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid,methanesulfonic acid, ethanesulfonic acid, 1,2-ethane-disulfonic acid,2-hydroxyethanesulfonic acid, benzenesulfonic acid,4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid,4-toluenesulfonic acid, camphorsulfonic acid,4-methylbicyclo[2.2.2]-oct-2-ene-1-carboxylic acid, glucoheptonic acid,3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid,lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoicacid, salicylic acid, stearic acid, muconic acid, and the like. A“pharmaceutically acceptable salt” of a compound also includes saltsformed when an acidic proton present in the parent compound is eitherreplaced by a metal ion, e.g., an alkali metal ion, an alkaline earthion, or an aluminum ion; or coordinates with an organic base such asethanolamine, diethanolamine, triethanolamine, tromethamine,N-methylglucamine, and the like.

“Pharmaceutically acceptable vehicle” means a carrier or inert mediumused as a solvent (or diluent) in which the medicinally active agent isformulated and or administered.

The terms “pro-drug” and “prodrug” are used interchangeably herein andrefer to any compound which releases an active parent drug according toFormula 1, or a pharmaceutically acceptable salt or solvate of Formula1, in vivo when such prodrug is administered to a mammalian subject.Prodrugs of a compound of Formula 1 are prepared by modifying one ormore functional group(s) present in the compound of Formula 1 in such away that the modification(s) may be cleaved in vivo to release theparent compound. Prodrugs include compounds of Formula 1 wherein ahydroxy group in a compound of Formula 1 is bonded to any group that maybe cleaved in vivo to regenerate the free hydroxyl, aliphatic alcohol,amino, or sulfhydryl group, respectively. Examples of prodrugs include,but are not limited to, esters (e.g., acetate, formate, glycol andbenzoate derivatives of Formula 1) and the like. For example, thecompound according to Formula 1 that is methyl3-(4-hydroxyphenoxy)-3-methyl-butanoate can be reacted under acidicconditions with 2-hydroxybenzoic acid to produce,[4-(3-methoxy-1,-1-dimethyl-3oxo-propoxy)2-hydoxybenzoate an esterprodrug that will be hydrolyzed to 2-hydroxybenzoic acid and thestarting compound by esterase enzymes in tissues. The transformationfrom prodrug to a compound of Formula 1, or a pharmaceuticallyacceptable salt or solvate thereof, may occur by various mechanisms,such as via hydrolysis in blood. A prodrug of the compound of Formula 1may be formed in a conventional manner according to methods known in theart. A thorough discussion of prodrugs is provided by V. Stella inPro-drugs as Novel Delivery Systems, Vol. 14 of the A.C.S. SymposiumSeries (Stella (1975)), and in Bioreversible Carriers in Drug Design(Roche (1987)), both of which are incorporated herein by reference.

“Pro-inflammatory cytokine” refers to a type of cytokine (i.e. a proteinsignaling molecule) that is secreted from leukocytes, mononuclear andother non-leukocyte cell types; such as but not limited epithelialcells, that promote inflammation by their biological effect on othercells and tissue in mammalian organisms. Non-limiting examples ofpro-inflammatory cytokines are: Interleukin 1 (IL-1; IL-la & IL-1b),Interleukin 6 (IL-6), Interleukin 13 (IL-13), Tumor Necrosis Factoralpha (TNF-alpha), Interferon gamma (IFN-gamma), and Interleukin 8(IL-8).

The term “prophylaxis” of a state, disorder, disease or condition asused herein refers to prevention of the appearance of clinical symptomsof the state, disorder, disease or condition developing in a patientthat is predisposed to the state, disorder, disease, or condition.

“Protecting group” refers to a moiety, with the exception of alkylgroups, that when attached to a reactive group in a molecule masks,reduces, or prevents that reactivity. Examples of protecting groups canbe found in T. W. Greene and P. G. M. Wuts, Protective Groups in OrganicSynthesis, 3rd edition, John Wiley & Sons, New York, 1999, and Harrisonand Harrison et al., Compendium of Synthetic Organic Methods, Vols. 1-8(John Wiley and Sons, 1971-1996), which are incorporated by referenceherein in their entirety. Representative hydroxy protecting groupsinclude acyl groups, benzyl and trityl ethers, tetrahydropyranyl ethers,trialkylsilyl ethers, and allyl ethers. Representative amino protectinggroups include, formyl, acetyl, trifluoroacetyl, benzyl,benzyloxycarbonyl (CBZ), tert-butoxycarbonyl (Boc), trimethyl silyl(TMS), 2-trimethylsilyl-ethanesulfonyl (SES), trityl and substitutedtrityl groups, allyloxycarbonyl, 9-fluorenylmethyloxycarbonyl (FMOC),nitro-veratryloxycarbonyl (NVOC), and the like.

“Corresponding protecting group” means an appropriate protecting groupcorresponding to the heteroatom (i.e., N, O, P, or S) to which it isattached.

As used herein the term “physiological pH” means a pH level of between7.0-7.9. The pH is defined as the negative log₁₀ of the hydrogen ionconcentration expressed in mol/L. A negative logarithrnic scale is usedbecause the numbers are all less than 1, and vary over a wide range. ThepH value is most accurately determined by measuring potential differencebetween electrodes placed in examined and reference solutions of knownpH or between measurement (glass) electrode and reference (calomel orsilver chloride) electrode also known as a pH meter.

“Signal transduction” or “signaling pathway activity” refers to abiochemical causal relationship generally initiated by a protein-proteininteraction such as binding of a biological active factor to a receptor,resulting in transmission of a signal from one portion of a cell toanother portion of a cell. In general, the transmission can involvespecific phosphorylation of one or more tyrosine, serine, or threonineresidues on one or more protein components such as enzymes ortranscription factors (i.e. intracellular secondary messengers) in theseries of reactions causing signal transduction (often referred to as acascade) that results in measurable changes to the cell. Penultimatecellular processes typically include nuclear events, resulting in achange in gene expression. Terminal events of signal transductioncascade result in changes in cellular activity such as but not limitedto, alterations in protein products produced and/or secreted by thecell, changes in cellular behavior characteristics of division,motility, adherence, etc.

“Stereoisomer” means molecules that have the same molecular formula,molecular weight and sequence of bonded atoms (constitution), but differin the three-dimensional orientations of their atoms in space. Bydefinition, molecules that are stereoisomers of each other represent thesame structural isomer. The chemical definitions and conventions usedherein generally follow S. P. Parker, Ed., McGraw-Hill Dictionary ofChemical Terms (1984) McGraw-Hill Book Company, New York; and Eliel, E.and Wilen, S., “Stereochemistry of Organic Compounds”, John Wiley &Sons, Inc., New York, 1994.

“A therapeutically effective amount” means the amount of a compoundthat, when administered to an individual for treating a disease, issufficient to effect such treatment for the disease, as defined below.The “therapeutically effective amount” will vary depending on thecompound, the disease and its severity or affected organ or tissue andthe age, weight, etc., of the individual to be treated.

“Tautomer” or “tautomeric form” means structural isomers of differentenergies which are interconvertible via a low energy barrier. Forexample, proton tautomers (also known as prototropic tautomers) includeinterconversions via migration of a proton, such as keto-enol andimine-enamine isomerizations. Valence tautomers include interconversionsby reorganization of some of the bonding electrons. The compounds of thepresent invention according to Formula 1 can exist in differenttautomeric states depending on the environment of the particularcompound, such as the acidity or alkalinity (i.e. pH) of the solution inwhich they are dissolved.

“Topical or topically applied” as used herein means the direct deliveryor application of the active drug ingredient such as compounds accordingto Formula 1 directly to the apical surface of a cell or to the exposedsurface of a tissue or organ. As used herein most often topicaladministration means application to epithelial surfaces such as theskin, eyes or mucous membranes to treat ailments by means of a spray,cream, ointment, shampoo, lotion, solution or other suitable deliverysolvent or vehicle at physiological pH. Various methods of topical drugdelivery such as; but not limited to mechanical application,instillation, inhalation, patches, and the like are known to thoseskilled in the art, are commonly used for topical application, and areimplicit in the concept of topical application as used herein.

“Treating” or “treatment” of a disease as used herein means inhibitingthe disease, i.e. arresting or reducing the pathophysiologic process orprocesses of the disease or its clinical symptoms; or relieving thedisease, i.e., causing regression of the pathophysiologic process orprocesses of disease or reducing the clinical manifestations of thepathophysiologic process or processes of the specific disease.

In some embodiments, a compound of the disclosure is present in acomposition as a salt. In some embodiments, salts are obtained byreacting a compound of the disclosure with acids. In some otherembodiments, pharmaceutically acceptable salts are obtained by reactinga compound of the disclosure with a base. In other embodiments, thecompounds are used as free-acid or free-base form in the manufacture ofthe compositions described herein. The type of salts, include, but arenot limited to: (1) acid addition salts, formed by reacting the freebase form of the compound with a pharmaceutically acceptable: inorganicacid, such as, for example, hydrochloric acid, hydrobromic acid,sulfuric acid, phosphoric acid, metaphosphoric acid, and the like; orwith an organic acid, such as, for example, acetic acid, propionic acid,hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid,lactic acid, malonic acid, succinic acid, malic acid, maleic acid,fumaric acid, trifluoroacetic acid, tartaric acid, citric acid, benzoicacid, 3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid,methanesulfonic acid, ethanesulfonic acid, 1,2-ethanedisulfonic acid,2-hydroxyethanesulfonic acid, benzenesulfonic acid, toluenesulfonicacid, 2-naphthalenesulfonic acid,4-methylbicyclo-[2.2.2]oct-2-ene-1-carboxylic acid, glucoheptonic acid,4,4′-methylenebis-(3-hydroxy-2-ene-1-carboxylic acid), 3-phenylpropionicacid, trimethylacetic acid, tertiary butylacetic acid, lauryl sulfuricacid, gluconic acid, glutamic acid, hydroxynaphthoic acid, salicylicacid, stearic acid, muconic acid, butyric acid, phenylacetic acid,phenylbutyric acid, valproic acid, and the like; (2) salts formed whenan acidic proton present in the parent compound is replaced by a metalion, e.g., an alkali metal ion (e.g. lithium, sodium, potassium), analkaline earth ion (e.g. magnesium, or calcium), or an aluminum ion. Insome cases, the lipid modulating compound described herein are reactedwith an organic base, such as, but not limited to, ethanolamine,diethanolamine, triethanolamine, methylamine, dimethylamine,trimethylamine, ethylamine, diethylamine, triethylamine,N-methylglucamine, dicyclohexylamine, tris(hydroxymethyl)methylamine. Inother cases, the compounds described herein form salts with amino acidssuch as, but not limited to, arginine, lysine, and the like. Acceptableinorganic bases used to form salts with compounds that include an acidicproton, include, but are not limited to, aluminum hydroxide, calciumhydroxide, potassium hydroxide, sodium carbonate, sodium hydroxide, andthe like.

In the scope of the embodiments, the compounds described herein includefurther forms of the compounds such as pharmaceutically acceptablesalts, solvates (including hydrates), amorphous phases, partiallycrystalline and crystalline forms (including all polymorphs), prodrugs,metabolites, N-oxides, isotopically-labeled, epimers, pure epimers,epimer mixtures, enantiomers including but not limited to singleenantiomers and enantiomeric diastereomers, meso compounds,stereoisomers, racemic mixtures and diasteroisomeric mixtures. Compoundsdescribed herein having one or more double bonds include cis/transisomers, E/Z isomers and geometric isomers.

In some embodiments, sites on the compounds disclosed herein aresusceptible to various metabolic reactions. Therefore incorporation ofappropriate substituents at the places of metabolic reactions willreduce, minimize or eliminate the metabolic pathways. In specificembodiments, the appropriate substituent to decrease or eliminate thesusceptibility of the aromatic ring to metabolic reactions is, by way ofexample only, a halogen, deuterium or an alkyl group. Examples of suchsubstituents can be found in Burger's Medicinal Chemistry, DrugDiscovery and Development, 8 Volume Set (Abraham (2010)) and in Foye'sPrinciples of Medicinal Chemistry (Lemke (2012)).

In some embodiments, sites on the compounds disclosed herein are notsusceptible to various metabolic reactions. Therefore incorporation ofappropriate substituents at or near or distant from the places of a lackof metabolic reactions will modulate, enhance, or maximize the metabolicpathways. In specific embodiments, the appropriate substituent(metabolic handle) to enhance, or maximize the susceptibility of thearomatic ring to metabolic reactions is, by way of example only, is aphenolic or methoxy or carboxylate group. Examples of such substituentscan be found in Burger's Medicinal Chemistry, Drug Discovery andDevelopment, 8 Volume Set (Abraham (2010)) and in Foye's Principles ofMedicinal Chemistry (Lemke (2012)).

Throughout the specification, groups and substituents thereof can bechosen by one skilled in the field to provide stable moieties andcompounds.

Synthesis of the Compounds

In general, compounds of Formula I may be prepared using a number ofmethods known in the chemical arts, particularly in light of thedescription contained herein, in combination with the knowledge of theskilled artisan. Various starting materials, intermediates, and reagentsmay be purchased from commercial sources or made according to literaturemethods or adaptations thereof. Although other reagents, compounds ormethods can be used in practice or testing, generalized methods for thepreparation of the compound of Formula I are illustrated by thefollowing descriptions and reaction Schemes. The methods disclosedherein, including those outlined in the Schemes, descriptions, andExamples are for intended for illustrative purposes and are not to beconstrued in any manner as limitations thereon. Various changes andmodifications will be obvious to those of skill in the art given thebenefit of the present disclosure and are deemed to be within the spiritand scope of the present disclosure as further defined in the appendedclaims.

Although specific embodiments of various aspects of the invention willbe described with reference to the Schemes, Preparations and/orExamples, it should be understood that such embodiments are by way ofexample only and are merely illustrative of a small number of the manypossible specific embodiments which can represent applications of theprinciples of the present disclosure. The starting materials used forthe synthesis of compounds described herein can be obtained fromcommercial sources, such as Aldrich Chemical Co. (Milwaukee, Wis.),Sigma Chemical Co. (St. Louis, Mo.), or the starting materials can besynthesized. The compounds described herein, and other related compoundshaving different substituents can be synthesized using techniques andmaterials known to those of skill in the art, such as described, forexample, in March's Advanced Organic Chemistry: Reactions, Mechanisms,and Structure (Smith (2013)), Design and Strategy in Organic Synthesis(Hanessian (2013)) Greene's Protective Groups in Organic Synthesis (Wuts(2006)) and Fiesers' Reagents for Organic Synthesis (Volumes 1-27) (Ho(2013)), each of which are incorporated by reference in their entirety.

General methods for the preparation of the compounds as disclosed hereinmay be derived from known reactions in the field, and the reactions maybe modified by the use of appropriate reagents and conditions, as wouldbe recognized by the skilled person, for the introduction of the variousmoieties found in the formulae as provided herein.

The intermediate products described can be recovered by extraction,evaporation, or other techniques known in the art. The crude materialsmay then be optionally purified by chromatography, HPLC,recrystallization, trituration, distillation, or other techniques knownin the art. In the discussions below, the following abbreviations wereused: EtOH (ethanol) NaOH (sodium ethoxide), DMSO (dimethylsulfoxide),MOM (methoxymethyl), THF (tetrahydrofuran), Dess-Martin (Dess-MartinPeriodinane) and TBS (tert-butyldimethylsilyl).

As would be appreciated by those skilled in the art, some of the methodsuseful for the preparation of such compounds, as discussed above, mayrequire protection of a particular functionality, e.g., to preventinterference by such functionality in reactions at other sites withinthe molecule or to preserve the integrity of such functionality. Theneed for, and type of, such protection is readily determined by oneskilled in the art, and will vary depending on, for example, the natureof the functionality and the conditions of the selected preparationmethod. Methods of introducing and removing protecting groups are wellknown to those of ordinary skill in the art and are described inGreene's Protective Groups in Organic Synthesis (Wuts (2006)). Alternatereagents, starting materials, as well as methods for optimizing oradapting the procedures described herein would also be readilydetermined by one skilled in the art.

The C═O Analogs

The traditional method of benzimidazole synthesis based on thecondensation of a suitably substituted alpha carbonyl acid chloride witha suitably substituted aromatic 1,2 diamine to provide the correspondingbenzimidazole can be used to synthesize compounds of this class, such asthose in Table 1, as shown in Scheme 1 below.

Similar pathways are available using appropriate activated estersinstead of the acid chloride as well as a version with a protected alphacarbonyl group.

An umpolung methodology can also provide this class of analogs directlyfrom a chlorinated benzimidazole as shown in Scheme 2. Such substitutedchloroimidazoles are widely available commercially.

The CHOH Analogs

Sodium Borohydride reduction of the C═O analogs prepared via Schemes 1or 2 leads directly to the CHOH analogs, including but not limited tothose listed in Table 2, as shown in Scheme 3. Other hydride reducingagents are envisaged such as one of the Selectides (L N or K Selectide)or LAH. Asymmetric reduction with a suitable optically active borane oraluminium hydride reagent can give the individual enantiomers.

The C(alkyl)OH Analogs

The traditional method of carbonyl alkylation with the action MethylLithium or Methyl Magnesium Bromide on the C═O analogs available fromSchemes 1 or 2 can be used to synthesize compounds of this class,including but not limited to those shown in Table 3, as shown in Scheme4 below.

Enantiomers are available from chiral HPLC methods.

The S═O Analogs

The methodology used to synthesize esomeprazole can be used to synthesisa wide range of S═O analogs, including but not limited to those shown inTable 4, as indicated below in Scheme 5 (Kohl et al (1992)).

Rather than going through a lengthy separation protocol involvingdiastereomeric derivatives of these sulfoxides an asymmetric synthesisof the enantiomeric analogs starting from the S precursor is moreefficient, including but not limited to those shown in Table 5, asindicated below in Scheme 6 (Lindberg et al (1998), Cotton et al(1998)).

Reversing the configuration from D-diethyl tartrate to L-diethyltartrate reverses the absolute configuration of the products, includingbut not limited to those shown in Table 6, as indicated below in Scheme7 (Lindberg et al (1998), Cotton et al (1998)).

The SO2 Analogs

Full oxidation of the S analogs gives the sulfones, including but notlimited to those shown in Table 7, as indicated below in Scheme 8. Otheroxidation conditions such as sodium periodate are envisaged.

The modular syntheses of Schemes 1 through 8 can all be adapted toautomated synthesis platforms, focused library platforms, solid phaseorganic synthesis platforms, combinatorial chemistry platforms,microwave chemistry platforms, DNA encoded library platforms and othermodern variants of synthetic organic chemistry suitable for highthroughput.

The following Tables 1 through 7 illustrate the analogs prepared.

TABLE 1 Structure

  1)

  2)

  3)

  4)

  5)

  6)

  7)

  8)

  9)

  10)

  11)

  12)

  13)

  14)

  15)

  16)

  17)

  18)

  19)

  20)

  21)

  22)

  23)

  24)

  25)

  26)

  27)

  28)

  29)

  30)

TABLE 2 Structure

  31)

  32)

  33)

  34)

  35)

  36)

  37)

  38)

  39)

  40)

  41)

  42)

  43)

  44)

  45)

  46)

  47)

  48)

  49)

  50)

  51)

  52)

  53)

  54)

  55)

  56)

  57)

  58)

  59)

  60)

TABLE 3 Structure

  61)

  62)

  63)

  64)

  65)

  66)

  67)

  68)

  69)

  70)

  71)

  72)

  73)

  74)

  75)

  76)

  77)

  78)

  79)

  80)

  81)

  82)

  83)

  84)

  85)

  86)

  87)

  88)

  89)

  90)

TABLE 4 Structure

  91)

  92)

  93)

  94)

  95)

  96)

  97)

  98)

  99)

  100)

  101)

  102)

  103)

  104)

  105)

  106)

  107)

  108)

  109)

  110)

  111)

  112)

  113)

  114)

  115)

  116)

  117)

  118)

  119)

  120)

TABLE 5 Structure

  121)

  122)

  123)

  124)

  125)

  126)

  127)

  128)

  129)

  130)

  131)

  132)

  133)

  134)

  135)

  136)

  137)

  138)

  139)

  140)

  141)

  142)

  143)

  144)

  145)

  146)

  147)

  148)

  149)

  150)

TABLE 6 Structure

  151)

  152)

  153)

  154)

  155)

  156)

  157)

  158)

  159)

  160)

  161)

  162)

  163)

  164)

  165)

  166)

  167)

  168)

  169)

  170)

  171)

  172)

  173)

  174)

  175)

  176)

  177)

  178)

  179)

  180)

TABLE 7 Structure

  181)

  182)

  183)

  184)

  185)

  186)

  187)

  188)

  189)

  190)

  191)

  192)

  193)

  194)

  195)

  196)

  197)

  198)

  199)

  200)

  201)

  202)

  203)

  204)

  205)

  206)

  207)

  208)

  209)

  210)

Methods of Inhibition of CXCL10 Production by Cells, Tissues and Organs.

The compounds and compositions described herein inhibit the geneexpression and production of CXCL10 by various types of mammalian cells,tissues and organs. Non-limiting examples are inhibition of productionof CCXL10 by keratinocytes, bronchial epithelial cells, peripheral bloodmonocyte cells, full thickness epidermal tissue and ocular retina underconditions known to increase CXCL10 gene expression and subsequentrelease CXCL10 protein into the cellular or tissue milieu, as measuredby various assays including immune based protein quantitation.

Methods of Treatment

The production of CXCL10 is induced in several cell and tissue typesduring the course of many diseases and exhibits pleiotropic effects on awide range of biological processes including immunity, angiogenesis andorgan-specific metastasis of cancers. Increased levels of CXCL10 incells, tissues and blood have been shown to contribute directly todisease pathogenesis and progression. Production of CXCL10 has beenshown to be directly contributory to the pathogenesis diseases affectingmany organs systems.

This disclosure provides methods for treating CXCL10 mediated diseasesor conditions selected from the group consisting of: AcquiredImmunodeficiency Syndrome, Acute Kidney Injury, Acute RespiratoryDistress Syndrome, Alzheimer Disease, Arthritis, Asthma, Astrocytoma,Behcet Syndrome, Bone Marrow Diseases, Breast Neoplasms, Bronchitis,Bronchopulmonary Dysplasia, Candidiasis, Ductal Breast Carcinoma,Hepatocellular Carcinoma, Non-Small-Cell Lung, Renal Cell Carcinoma,Chorea, Chorioamnionitis, Colitis, Colonic Neoplasms, ColorectalNeoplasms, Corneal Neovascularization Coronary Disease, Crohn Disease,Cryoglobulinemia, Cystic Fibrosis, Dementia, Dengue, Dermatitis,Diabetes Mellitus, Diabetes Mellitus, Dry Eye Syndromes, Eczema,Encephalitis, Endometrial Neoplasms, Endometriosis, Epstein-Barr VirusInfections, Fatigue, Fibrosis, Glioma, Gliosis, Granuloma, Graves'Disease, HIV Infections, Hepatitis, Idiopathic Pulmonary Fibrosis,Inflammatory Bowel Diseases, Kidney Neoplasms, Lichen Planus, LiverCirrhosis, Lupus Erythematosus, Lupus Nephritis, Lymphoma, MacularDegeneration, Malaria, Melanoma, Malignant Melanoma, Multiple Sclerosis,Myasthenia Gravis, Mycoplasma Pneumonia, Myositis, Osteoarthritis,Pancreatitis, Parkinson Disease, Periodontal Disease,Polyradiculoneuropathy, Prader-Willi Syndrome, Pre-Eclampsia, ProstaticNeoplasms, Pruritus, Psoriasis, Pulmonary Emphysema, Pyelonephritis,Retinopathy, Sarcoidosis, Scleroderma, Sinusitis, Systemic Sepsis,Sjögren's Syndrome, Spondylitis, Still's Disease, Stomach Neoplasms,Stroke, Thyroiditis, Traumatic Brain Injury, Tuberculosis, UterineCervical Neoplasms, Uveitis, Wound degeneration.

An embodiment of this invention directed to a method of treating CXCL10mediated diseases in a patient in need of such treatment comprisingadministering to the patient a therapeutically effective amount of atleast one compound of formula (1), or a pharmaceutically acceptable saltor solvate thereof. The methods of treatment of this disclosure areespecially advantageous in treating diseases where CXCL10 binds to CXCR3receptor but are not limited to that binding occurring at physiologicalpH.

One embodiment of this invention is directed to a pharmaceuticalcomposition comprising at least one compound of Formula 1, or apharmaceutically acceptable salt or solvate thereof, in combination witha pharmaceutically acceptable carrier at physiological pH.

A preferred embodiment on this invention is directed to a topical routeof administration of a pharmaceutical composition comprising at leastone compound of formula (1), or a pharmaceutically acceptable salt orsolvate thereof, in combination with a pharmaceutically acceptablecarrier at physiological pH.

Another preferred embodiment of this disclosure is a method of treatinga CXCL10 mediated disease by parenteral administration of (a) atherapeutically effective amount of at least one compound of formula(1), or a pharmaceutically acceptable salt or solvate thereof, incombination with a pharmaceutically acceptable carrier at physiologicalpH.

In an embodiment of the present invention the compounds according toFormula 1 (or formula 2) may be used either simultaneously orsequentially in combination with a second compound such as, but notlimited to: non-steroidal anti-inflammatory drugs, such as aspirin,choline salicylate, celecoxib, acetaminophen, diclofenac, flurbiprofen,ibuprofen, indomethacin, ketoprofen, meclofenamate, mefenamic acid,nabumetone, naproxen, piroxicam, rofecoxib, salicylates, sulindac,tolmetin, and valdecoxib; immunomodulatory agents, such as methotrexate,azathioprine, mitoxantrone, cladribin, cyclophosphamide, tacrorimus,methotrexate, cyclosporine, and hydroxychloroquine; antimalarials, suchas chloroquine, quinine, amodiaquine, pyrimethamine, proguanil,mefloquine, atovaquone, primaquine, artemisinin, and halofantrine;antibiotics, such as sulfonamides, clindamycin, members of thetetracycline family (including minocycline and doxycycline),erythromycin, and dapsone; anti-TNF alpha agents, such as infliximab,adalimumab, certolizumab pegol, golimumab, thalidomide, lenalidomide,pomalidomide, and etanercept; anti-CD20 agents, such as but not limitedto: rituximab, obinutuzumab, Ibritumomab tiuxetan, and tositumomab;antidiarrheals, such as lidamidine, diphenoxylate, loperamide, andquercetin; antidepressants, such as amitriptyline, clomipramine, doxepinnortriptyline, and trimipramine; antipsychotics, such as droperidol,pimozide, chlorpromazine, thiothixene, loxapine, molindone, quetiapine,risperidone, sertindole, and zotepine; antifungals, such as clotimazole,flucisoconazole, abafungin, micafugin, terbinafine, ciclopirox, andtolnaftate; antihelminthics, such as mebendazole, levamisole, abamectin,and suramine; T lymphocyte activation inhibitors, such as cyclosporine,voclosporin, peroxynitrite, and dasatinib; anti-IL-1 agents, such asanakinra and IL-1Ra; antihyperglycemic agents, such as insulin,glyburide, glipizide, pioglitazone, acarbose, nateglinide, andmetformin; glucocorticoids, such as methyl prednisolone, prednisolone,dexamethasone, betamethasone, fluticasone propionate, budesonide,flunisolide, mometasone furoate, triamcinolone acetonide, rofleponide,ciclesonide, and butixocort propionate; anti-cytokine/chemokinemonoclonal antibodies, such as basiliximab, daclizumab, and secukinumab;sex steroids and receptor modulators, such as progesterone, progestins,androgen, estrogen, mifepristone, and misoprostil; antiacid agents, suchas cimetidine, magnesium hydroxide, esomeprazole and calcium carbonate;anti-cellular surface receptor monoclonal antibodies directed againstcell surface receptors such as CCR1, CCR3, CXCR2, CXCR3, CCL4, CCR5,IL7Ra, and TSLPR; aminosalicylic acid derivatives such as sulfasalazineand mesalazine; anticholinergic agents, such as ipratropium, oxitropium,tiotropium, dextromethorphan, revatropate, pirenzepine, darifenacin,oxybutynin, mecamylamine, terodiline, tolterodine, trospium chloride,and solifenacin; adrenergic agonists, such as but not limited to:salmeterol, salbutamol, clonidine, oxymetazoline, and dolbutamine;cholinergic agonists, such as carbachol, epibatidine, galantamine,nicotine, and varenicline; corticosteroids, such as cortisone andhydrocortisone; antineoplastic chemotherapeutic agents, such ascisplatin cyclophosphamide, bleomycin, doxorubicin, etoposide, folinicacid, and vincristine; phosphodiesterase inhibitors, such asmesembrenone, rolipram, Ibudilast, piclamilast, luteolin, drotaverine,roflumilast, cilomilast, apremilast, and crisaborole; leukotrienepathway modulators, such as3-[3-butylsulfanyl-1-[(4-chlorophenyl)methyl]-5-propan-2-yl-indol-2-yl]-2,2-dimethyl-propanoicacid, baicalein, caffeic acid, curcumin, hyperforin, and zileuton;monoclonal antibodies directed against human immunoglobulins, such asomalizumab; adrenergic antagonists, such as alfluosin, idazoxan,labetalol, phentolamine, trazadone, propranolol, and atenolol; calciumchannel antagonists, such as amelodipine, nifedapine, verapamil,diltiazem, and mibefradil; dopamine agonists, such as aripiprazole,bromocriptine, bupropion, cabergoline, lisuride, and roxindole;serotonin agonists, such as cabergoline, cisapride, gepirone,lorcaserin, and naratriptan; dopamine antagonists, such as amoxipine,bromopride, butaclamol, eticlopride, olanzapine, tiapride, andziprasidone; serotonin antagonists, such as cyproheptadine, ketanserin,metergoline, methdilazine, oxetorone, and tropisetron; monoaminereuptake inhibitors, such as amineptine, citalopram, edivoxetine,hyperforin, mazindol, and viloxazine; protease inhibitors, such asamastatin, bestatin, and gabexate; histamine receptor antagonists, suchas acrivastine, brompheniramine, cetirizine, cimetidine, ciproxifan,clobenprobit, cyclizine, carebastine, cyproheptadine, ebastine,epinastine, efletirizine, fexofenadine, and thioperamide; antihypertriglycerides, such as fenofibrate, fibric acid, rosuvastatin,gemfibrozil, and omega-3-acid ethyl esters; HMG-CoA reductaseinhibitors, such as atorvastatin, fluastatin, lovastatin, andsimvastatin; retinoids such as etretinate, tretinoin, retinol, retinylpalmitate. So as to inhibit the biological activity of CXCL10 orotherwise aid in the treatment of the CXCL10 mediated disease orcondition undergoing treatment.

Pharmaceutical Compositions

Administration of the therapeutic agent may be by any suitable means. Insome embodiments, the one or more therapeutic agents are administered byoral administration. In some embodiments, the one or more therapeuticagents are administered by transdermal administration. In someembodiments, the one or more therapeutic agents are administered byinjection or intravenous infusion. In one embodiment, the one or moretherapeutic agents, at physiological pH, are administered topically to amucosal, dermal, or ocular tissue. In another embodiment, administrationof the compounds disclosed herein can be by drug eluting devises ormatrices so as provide a prolonged dosage of the compound over time.

If combinations of agents are administered as separate compositions,they may be administered by the same route or by different routes. Ifcombinations of agents are administered in a single composition, theymay be administered by any suitable route. In some embodiments,combinations of agents are administered as a single composition by oraladministration. In some embodiments, combinations of agents areadministered as a single composition by transdermal administration. Insome embodiments, the combinations of agent are administered as a singlecomposition by injection. In some embodiments, the combinations of agentare administered as a single composition topically.

In one embodiment of the present invention the compounds of Formula 1may contain asymmetric or chiral centers and, therefore, exist indifferent stereoisomeric forms. For example, 2,2,2-trifluoroethyl2-(4-methylphenoxy)propanoate is a compound according Formula 1 thatpossesses a chiral center at carbon atom number 8 and thus has twostereoisomer forms. It is intended that all stereoisomeric forms of thecompounds of Formula 1 form part of the present invention, including butnot limited to: diastereomers, enantiomers, and atropisomers as well asmixtures thereof, such as racemic mixtures. In addition, the presentinvention embraces all geometric and positional isomers. For example, ifa compound of Formula 1 incorporates a double bond or a fused ring, boththe cis- and trans-forms, as well as mixtures, are embraced within thescope of the invention. Both the single positional isomers and mixtureof positional isomers are also within the scope of the presentinvention.

In one embodiment of the present invention, compounds of Formula 1 mayexist in different tautomeric forms, and all such forms are embracedwithin the scope of the invention, as defined by the claims.

The dose and dosing regimens of the compound present in the inventionmay be adjusted to provide the optimum desired response in accordancewith methods and practices well known in the therapeutic arts. Forexample, a single bolus dose may be administered, or several divideddoses may be administered over time. The dose may also be proportionallyreduced or increased as indicated by the exigencies of the therapeuticsituation. The appropriate dosing regimen, the amount of each doseadministered and/or the intervals between doses will depend upon anumber of factors, including: the compound, the type of pharmaceuticalcomposition, the characteristics of the subject in need of treatment andthe severity of the condition being treated.

The dose of the compound will vary, but as a general guideline fordermatological administration, the compound will be present in adermatologically acceptable formulation in a therapeutically effectivedose in an amount of from about 0.0001 mg/kg to about 1000 mg/kg/bodyweight per day of a compound provided herein. The pharmaceuticalcompositions therefore should provide a dosage of from about 0.0001mg/kg/body weight to about 1000 mg/kg/body weight of the compound forsome conditions. In another embodiment of the present invention thepharmaceutical dosage unit forms are prepared to provide a preparationfor topical application containing 0.01 to 50 w/w %, and more typicallyfrom about 0.1 to 10 w/w %. In yet other embodiments the pharmaceuticaldosage unit forms are prepared to provide a preparation for topicalapplication containing from 0.01% to 30% (w/v) of the compounds.

In some embodiments, the formulation may be applied to the affected areafrom 1 to 6 times daily. A “dermatologically acceptable formulation” isone that may be applied to the skin or hair and will allow the drug todiffuse to the site of action.

In some embodiment the compounds can be formulated into topicallyapplied eye drops or gels using suitable salts or solvates thereof incombination with a pharmaceutically acceptable carrier. Preferredspecific pharmaceutical carriers are known to those skilled in the artthat facilitate penetration of the compounds to all regions of the eyesuch as but not limited to the: anterior segment, posterior segment,sclera, choroid and retina.

In some embodiments the compounds can be formulated into solid orsemi-solid matrices that serve to provide a reservoir of the compoundwithin a tissue or organ so as to provide a continuous supply of thecompound for treatment over days, weeks or months.

The skilled artisan can also be expected to readily determine themaximum tolerable dose, the therapeutically effective amount whichprovides a detectable therapeutic benefit to a patient, and the temporalrequirements for administering each agent to provide a detectabletherapeutic benefit to the patient. Accordingly, while certain dose andadministration regimens are exemplified herein, these examples in no waylimit the dose and administration regimen that may be provided to apatient in practicing the present invention.

The determination of optimal dosages for a particular patient iswell-known to those skilled in the art. Certain non-limiting examples ofpharmaceutically acceptable vehicles suitable for topical administrationinclude propylene glycol:transcutanol:ethanol (20:20:60, v/v/v) andpropylene glycol:ethanol (30:70, v/v). In some embodiments, the compoundof Formula 1 may be present at concentrations of between about 0.1% toabout 10% (w/v).

In another embodiment, the medicinal and cosmetic formulationscontaining the compound and any additional therapeutic agents willtypically be packaged for retail distribution (i.e. an article ofmanufacture or a kit). Such articles will be labeled and packaged in amanner to instruct the patient how to use the product. Such instructionswill include the condition to be treated, duration of treatment, dosingschedule, etc. The compound(s) of Formula 1 may also be admixed with anyinert carrier and utilized in laboratory assays in order determine theconcentration of the compounds within the serum, urine, etc., of thepatient as is known in the art. The compound may also be used as aresearch tool.

While the invention has been described in connection with specificembodiments thereof, it will be understood that it is capable of furthermodifications and this application is intended to cover any variations,uses, or adaptations of the invention following, in general, theprinciples of the invention and including such departures from thepresent disclosure as come within known or customary practice within theart to which the invention belongs. The following examples andbiological data are being presented in order to further illustrate theinvention. This disclosure should not be construed as limiting theinvention in any manner.

For all of the foregoing embodiments, each embodiment disclosed hereinis contemplated as being applicable to each of the other disclosedembodiments. Those skilled in the art will readily appreciate that thespecific Experimental Details which follow are only illustrative of theinvention as described more fully in the claims which follow thereafter.

Examples 1. Inhibition of CXCL10 Gene Expression and CXC110 ProteinProduction in Human Cells and Tissues

To determine the ability of compounds, according to Formula 1 to inhibitCXCL10 gene expression, protein production and secretion usingfunctional assays we used cultivated normal human keratinocytes,bronchial epithelial cells, human peripheral blood mononuclear cells andreconstituted full thickness human epithelium tissue. These cells andtissue were treated with stimulants known to induce gene expression,production and secretion of CXCL10. Various compounds of the inventionwere tested for their ability to inhibit CXCL10 gene expression and/orproduction of the CXCL10 protein. The gene expression and production ofCXCL10 protein were determined by well-known methods as described below.

Human keratinocyte cells (NEHK) from healthy volunteers were obtainedand cultivated at 1×106 cells/ml using 6 well plates in NEHK-GM mediaaccording to supplier's protocol (MatTek Corporation; Ashland, Mass.).Human bronchial epithelial cells (NHBE) were obtained and cultivated at1×106 cells/ml using 6 well plates in NHBE-GM media according tosupplier's protocol (MatTek Corporation; Ashland, Mass.). Humanperipheral blood monocytic cells (PBMC) were obtained by Ficol densitygradient centrifugation and cultivated at 1×106 cells/ml using 6 wellplates in cultured at 1×106 cells/ml in RPMI-1640 medium (GIBCO® Inc.Carlsbad, Calif., USA) supplemented with 20% fetal bovine serum and 1%streptomycin/penicillin according to standard protocol(ThermoFisher-Invitrogen; Carlsbad Calif.).

The EpiDermFT (EFT-400) a reconstructed full thickness human epithelialtissue, (MatTek Corporation; Ashland, Mass.) was used to evaluate CXCL10gene expression and CXCL10 protein production and secretion from intacthuman tissue. The tissues were cultivated and matured according tomanufacturer's protocol. After maturation of the EpiDermFT tissues wasachieved, the tissues were then used for testing.

Human cell suspensions and tissues without stimulation agents known toinduce CXCL10 production were used as a baseline control for theexperiments. Neither the unstimulated cells nor the tissues expressedthe CXCL10 gene or produced CXCL10 protein in measurable amounts. Thecell cultures and tissues were treated with various exemplar compoundsaccording to Formula 1 at several concentrations for 8 hours prior tostimulation by CXCL10 inducing agents. The tissue samples were alsotested with various exemplar compounds according to Formula 1 byapplying the test compound to the apical surface of the tissue for 8hours prior to stimulation by CXCL10 inducing agents.

The cell cultures of NHEK, NHBE, and PBMC were stimulated with variousknown stimulators of CXCL10 production that consisted of: Poly [I:C] 10ug/ml (Invivogen; San Diego, Calif.) (see FIG. 1a and FIG. 1b ),Lipopolysaccharide (LPS) 10 ug/ml (Invivogen; San Diego, Calif.) (seeFIGS. 2a and 2b ), Interferon gamma (IFN-7) 10 ng/ml (Invivogen; SanDiego, Calif.) (see FIGS. 3a and 3b ). The EFT-400 tissues werestimulated with Interferon gamma (IFN-7) 10 ng/ml plus Tumor NecrosisFactor alpha (TNF-α) 10 ng/ml (Invivogen; San Diego, Calif.) which actsynergistically to maximally induce CXCL10 (see FIGS. 4a and 4b ).

Measurement of CXCL10 gene expression was determined from total RNAextracted from cell pellets and tissues after incubation by RNAseqquantitation method standardized using expression of housekeeping genesas reference standard. CXCL10 protein production was measured in thecell and tissue culture supernatants after incubation using the CXCL10ELISA kit (R&D Systems; Minneapolis, Minn.). The results of these assaysdemonstrated that induction of CXCL10 gene expression, subsequentprotein expression and secretion was significantly inhibited by thecompounds.

2. In Vivo Inhibition of CXCL10 Gene Expression and AssociatedRetinopathy in Rats

CXCL10 expression is associated with age related and diabetes inducedretinopathy. A hallmark of these retinopathies is retinal vascularleakage (i.e. increased retinal vascular permeability) thought toinduced by CXCL10. To test the in vivo effect of the compounds ontreatment of retinopathies associated with increased CXCL10 levels,diabetes was induced in rats by treatment with Streptozotocin (STZ)according established methods (Akbarzadeh et al, J Clin Biochem. 2007September; 22(2): 60-64) Testing began once the diabetes had beenestablished in the animals. Compounds were formulated into a topicallyapplied eye drop solution containing 1-5% of compounds 91 and 108 alongwith ethylene glycol, sodium lauryl sulfate, hyaluronic acid, andsterile water at pH 7.0. The drops containing the test compound wereapplied (20 μL) three times per day in one eye and drops that did notcontain the test compound were applied to the other eye in similarfashion for 30 days. CXCL10 expression was measured in retina tissuehomogenate and retinal vascular permeability was assayed using themeasurement of leakage of Evans Blue dye from retinal vessels, awell-established method (Invest Ophthalmol Vis Sci 2001 March; 42:789-794). The treatment of the animals with the compounds demonstrated asignificant reduction in the amount of CXCL10 produced by the retina anda reduction of the retinal vascular permeability as compared to thecontrol eye (FIG. 5a-b ).

3. Clinical Treatment of Dermatitis/Eczema

To test the effect of the compounds on treatment of human dermatologicdisorders associated with increased CXCL10 production by keratinocytes,Compound 97 and Compound 98 were formulated with stearic acid, lanolin(anhydrous), mineral oil and triethanolamine into a topically appliedcream at a concentration of 7%. The compounds in the cream base wereapplied topically twice per day to human subjects suffering fromdermatitis in an area of the skin with active skin lesions for 3-4weeks. The control was the topical cream base composition without theactive compounds. Outcome was measured by an experienced clinicalobserver as assessed by visible reduction or disappearance of thelesions as assessed in the treated area vs the control area treated onlywith the cream base alone using Investigator's Global Assessment (IGA)scale. The study revealed the compounds had a pronounced effect onreducing the dermatitis/eczema (FIG. 6).

4. In Vivo Inhibition of CXCL10 Expression and Uvetitis

CXCL10 expression is associated with ocular inflammation (uveitis).Therefore, to test the effect of the compounds to treat uveitis, C57BL/6mice were induced to have experimental autoimmune uveitis (EAU) usingstandard methods Caspi R R et al. Journal of immunology (Baltimore, Md.1988; 140: 1490-1495). Briefly, EAU was induced by active immunizationwith bovine interphotoreceptor retinoid-binding protein (IRBP), using150 ug in a 0.2 ml emulsion (1:1 v/v with complete Freund's adjuvant(CFA) containing Mycobacterium tuberculosis strain H37RA (2.5 mg/mL).EAU is an established preclinical animal model for assessment ofimmunotherapeutic efficacy treatments for posterior uveitis. The courseand severity of the induced ocular inflammation can be evaluated andobjectively scored using Topical Endoscope Fundus Imaging (TEFI) asdescribed by Paques et al. (Invest Ophthalmol Vis Sci. 2007;48:2769-2774) and modified by Copland et al. (Invest Ophthalmol Vis Sci.2008; 49:5458-5465). The time course and severity of the induced uveitisin the mice (treated vs vehicle control) was followed over a 3-weekperiod and TEFI was measured. Experiment was performed in triplicate fortreatment verses negative control vehicle only. The study revealed thatthe compounds tested had a pronounced effect on reducing the EAU.

The results of the experiments and clinical tests herein demonstratethat compounds according to Formula 1 inhibit the production of CXCL10from human cells and tissues. In addition, they demonstrate a clinicaltherapeutic effect in subjects suffering from dermatologic disorder andin an established animal model of a retinal disorder associated withproduction of CXCL10. Therefore, the compounds according to Formula 1are useful in to reduce the production of CXCL10 in human cells andtissues induced by various stimulants and for treatment of variousdisorders associated with increased CXCL10 production such as but notlimited to dermatitis and retinopathy.

LIST OF REFERENCES

-   D. J. Abraham et al. (2010) “Burger's Medicinal Chemistry, Drug    Discovery and Development” Wiley, 7th Edition-   T. L. Lemke et al. (2013) “Foye's Principles of Medicinal Chemistry”    Wiley, 7th Edition-   M. B. Smith (2013) “March's Advanced Organic Chemistry: Reactions,    Mechanisms, and Structure” Wiley, 7th Edition-   S. Hanessian et al. (2013) “Design and Strategy in Organic    Synthesis” Wiley-VCH, 1st Edition-   P. G. M. Wuts et al. “Greene's Protective Groups in Organic    Synthesis” Wiley-Interscience, 4th Edition-   T. L. Ho “Fiesers' Reagents for Organic Synthesis” Wiley, Volume 27    Edition-   Kohl B, Sturn E, Senn-Bilfinger J et al, J. Med. Chem, 35, 1049-1053    (1992)-   Lindberg P L, Von Unge S, U.S. Pat. No. 5,714,504 (1998)-   Cotton H et al, WO 9854171 (1998)

1. A method of treating a disorder associated with increased expressionof CXCL10 in a human subject comprising administering to a patient inneed thereof an effective amount of a composition comprising apharmaceutically acceptable carrier and a compound, pharmaceuticallyacceptable salt, ester, or prodrug of Formula 1 at physiological pH

wherein: X is selected from CH or N; Y is selected from CH₂, CHOH,C(optionally substituted C₁ to C₈ straight chain or branched chainalkyl)OH, C═O, NH, N-optionally substituted C₁ to C₈ straight chain orbranched chain alkyl, NCO-optionally substituted C₁ to C₈ straight chainor branched chain alkyl, S, S═O, SO₂; R₁, R₂, R₃, R₄, R₅, R₆, and R₇ areindependently selected from: H; OH; F; Cl; Br; I; (halogen)alkyl,optionally substituted C₁ to C₈ straight chain or branched chain alkyl;optionally substituted C₁ to C₈ cycloalkyl; heterocycloalkyl;alkylheterocycloalkyl; optionally substituted C₁ to C₈ alkenyl;optionally substituted C₁ to C₈ alkynyl; optionally substituted aryl;optionally substituted alkylaryl; optionally substituted heteroaryl;optionally substituted alkylheteroaryl; O-alkyl; O-optionallysubstituted alkyl, O-cycloalkyl; O-alkylcycloalkyl; O-aryl; O-optionallysubstituted aryl; alkyl-O-aryl; alkyl-O-optionally substituted aryl;C(O)-aryl; C(O)-optionally substituted aryl; CH₂C(O)-aryl;CH₂C(O)-optionally substituted aryl; O-(halogen)alkyl, whereinoptionally substituted alkenyl, if present, may have one or more doublebond and each double bond may independently be cis or trans, E or Z, acis/trans mixture or an E/Z mixture, or adjacent substituents R₁ and R₂,R₂ and R₃, R₄ and R₅, R₅ and R₆, R₆ and R₇ may form a saturated orunsaturated 5 membered or 6-membered or 7 membered carbocyclic orheterocyclic ring, wherein if at least one asymmetric center is presentthe compound may be in the form of a racemic mixture, a singleenantiomer, a diastereoisomeric mixture, an enantiomeric diastereomer, ameso compound, a pure epimer, or a mixture of epimers thereof, andwherein a hydrogen, several hydrogens or all hydrogens may be replacedwith deuterium, or a pharmaceutically acceptable salt, ester or prodrugform thereof.
 2. The method according to claim 1, wherein the humandisorder is selected from any one of: Acquired ImmunodeficiencySyndrome, Acute Kidney Injury, Alzheimer Disease, Arthritis, Asthma,Astrocytoma, Behcet Syndrome, Breast Neoplasms, Bronchitis, ChronicObstructive Pulmonary Disease, Crohn Disease, Cryoglobulinemia, CysticFibrosis, Dermatitis, Diabetic Retinopathy, Dry Eye Syndrome,Encephalitis, Endometriosis, Fibrosis, Gliosis, Hepatitis,Hepatocellular Carcinoma, Idiopathic Pulmonary Fibrosis, InterstitialCystitis, Kidney Neoplasm, Lichen Planus, Liver Biliary Cirrhosis, LupusErythematosus, Lupus Nephritis, Lymphocytic Choriomeningitis, MacularDegeneration, Melanoma, Multiple Sclerosis, Myasthenia Gravis, Myositis,Non-Small-Cell Carcinoma of the Lung, Non-Renal Cell Carcinoma,Osteoarthritis, Pancreatitis, Parkinson Disease, Polyradiculoneuropathy,Prader-Willi Syndrome, Pre-Eclampsia, Psoriasis, Renal Cell Carcinoma,Retinopathy of Prematurity, Sarcoidosis, Scleroderma, Sjogren'sSyndrome, Spondylitis, Ulcerative Colitis, Uveitis, or Wounddegeneration.
 3. The method according to claim 2, wherein the humandisorder is Diabetic Retinopathy.
 4. The method according to claim 2,wherein the human disorder is uveitis.
 5. The method according to claim2, wherein the human disorder is macular degeneration.
 6. The methodaccording to claim 2, wherein the human disorder is dermatitis.
 7. Themethod according to claim 2, wherein the human disorder is interstitialcystitis.
 8. The method of claim 1, wherein the compound according toclaim 1 is administered simultaneously or sequentially in combinationwith other compounds selected from the group consisting of non-steroidalanti-inflammatory drugs; immunomodulatory agents; antimalarials;antibiotics; anti-TNF alpha agents; anti-CD20 agents; antidiarrheals;Bioactive peptides; corticosteroids; antidepressants; antipsychotics;antifungals; antihelminthics; T lymphocyte activation inhibitors;anti-IL-1 agents; antihyperglycemic agents; glucocorticoids;anti-cytokine/chemokine monoclonal antibodies; sex steroids and receptormodulators; antiacid agents; anti-cellular surface receptor monoclonalantibodies directed against cell surface receptors; aminosalicylic acidderivatives; adrenergic agonists; cholineric agonists; corticosteroids;antineoplastic chemotherapeutic agents; phosphodiesterase inhibitors;leukotriene pathway modulators; monoclonal antibodies directed againsthuman immunoglobulins; adrenergic antagonists; calcium channelantagonists; dopamine agonists; serotonin agonists; dopamineantagonists; serotonin antagonists; monoamine reuptake inhibitors;protease inhibitors; histamine receptor antagonists; antihypertriglycerides; HMG-CoA reductase inhibitors; and retinoids. 9.(canceled)
 10. The compound according to claim 1, wherein the compoundis selected from the group consisting of Structure

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11. The compound according to claim 1, wherein the compound is selectedfrom the group consisting of


12. The compound according to claim 1, wherein the compound is selectedfrom the group consisting of


13. The compound according to wherein the compound is selected from thegroup consisting of


14. The compound according to claim 1, wherein the compound is selectedfrom the group consisting of


15. The compound according to claim 1, wherein the compound is selectedfrom the group consisting of


16. The compound according to claim 1, wherein the compound is selectedfrom the group consisting of

17.-20. (canceled)